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Linz Winter Workshop 2016

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29 January - 1 February 2016 ...  more of Linz Winter Workshop 2016 (Titel)

Schindl Awarded Habilitation (Postdoctoral Teaching Certification)

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Paper of the week:

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[2016/01/05] The cover of Science Signaling illustrates the discovery of a calcium-accumulating region in the calcium selective ion-channel Orai1. The publication titled "A calcium-accumulating region, CAR, in the channel Orai1 enhances Ca2+ permeation and SOCE-induced gene transcription," describes how the channel adjusts to environmental calicum levels in tissues and the blood stream to maintain calcium driven gene regulation. ...  more of Paper of the week: (Titel)

Tiemann-Boege Hunts Down Early Gene Material Mutations

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[2015/12/23] Tiemann-Boege's working group develops measurement method for early detection of gene mutations. The quality of genetic material is not only subject to maternal age - paternal age also has a decisive influence... ...  more of Tiemann-Boege Hunts Down Early Gene Material Mutations (Titel)

Romanin’s Reseach Group Successfully Procures FWF Funding

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[2015/12/17] Prof. Romanin from the Institute for Biophysics is extremely proud that his working group managed to attract a total of six new FWF projects... ...  more of Romanin’s Reseach Group Successfully Procures FWF Funding (Titel)

Tiemann-Boege: Winner of the OOE Forscherinnen Award 2015, Category “Gesundheit”

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[2015/05/20] Top recognition for the Johannes Kepler University (JKU) Linz at the 2015 Upper Austrian Research Awards for Women... ...  more of Tiemann-Boege: Winner of the OOE Forscherinnen Award 2015, Category “Gesundheit” (Titel)

Pohl Group resolves mystery of cellular water transport

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[2015/03/25] Ancient Greek philosopher Heraclitus believed "Panta rhei" – "everything flows", which is especially true when it comes to the water in our bodies. The question, however, has always been just how... ...  more of Pohl Group resolves mystery of cellular water transport (Titel)


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Membrane Transport, Peter Pohl

The research foci include transport of water, protons, other small molecules, and proteins through biological membranes. We thus exploit (i) primary cells or tissues, (ii) cultured cells that are genetically modified to overexpress the protein of interest or (iii) planar lipid bilayers or lipid vesicles both reconstituted with the purified membrane transporter. Purification occurs by overexpressing E.coli cells or yeast cells using affinity and size-exclusion chromatography.
      Our work aims to unravel molecular transport mechanisms. We specifically want to understand (i) the major determinants of single file water transport in potassium channels, aquaporins, artificial nanopores, (ii) the principles of water flux through secondary active transporters, (iii) the structural-functional relationship which allows the protein translocase to facilitate the passage of large molecules while maintaining the membrane barrier for small molecules, (iv) the mechanisms of lateral proton migration between two membrane proteins.

The proteins under investigation are genetically or chemically modified to test the functional importance of certain residues and to introduce fluorescent labels. We monitor protein function by implementing a wide variety of methods including: recordings of current through single channels and through channel ensembles, streaming and boundary potential measurements, particle electrophoresis, stopped- flow spectroscopy, fluorescence correlation spectroscopy, fluorimetry, confocal fluorescence microscopy, and scanning electrochemical microscopy.