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Tiemann-Boege Awarded Pilgerstorferpreis

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[2016/07/07] Assoc. Prof. Dr. Irene Tiemann-Boege was awarded the Pilgerstorferpreis from the Medical Society of Upper Austria.

Heißl awarded ÖAW Doc Scholarship

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[2016/03/04] Angelika Heißl's 3-year DOC fellowship granted by the Austrian Acadamy of Sciences (ÖAW) started on the 1 January 2016. The title is: "Meiotic recombination as a potential driver of microsatellite evolution". ...  more of Heißl awarded ÖAW Doc Scholarship (Titel)

Paper of the month:

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[2016/02/15] Cholesterol in the fight against allergies ...  more of Paper of the month: (Titel)

Biophysics Researchers Discover New Immune Protein Structure

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[2016/02/05] Scientists at the JKU Institute for Biophysics (a working group under a. Univ. Prof. Christoph Romanin) have deciphered a key function in the “Orai1” immune protein. ...  more of Biophysics Researchers Discover New Immune Protein Structure (Titel)

Nanopharmacological force sensing to reveal allosteric coupling in transporter binding sites

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[2016/01/26] Novel tools for direct measurement of the interaction forces between the serotonin transporter and the S- and R-enantiomers of citalopram on the single molecule level allows for the demonstration of two distinct populations of characteristic binding strengths which provide physical evidence for the existence of two binding sites that are allosterically coupled and exert reciprocal modulation. ...  more of Nanopharmacological force sensing to reveal allosteric coupling in transporter binding sites (Titel)

Tiemann-Boege Hunts Down Early Gene Material Mutations

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[2015/12/23] Tiemann-Boege's working group develops measurement method for early detection of gene mutations. The quality of genetic material is not only subject to maternal age - paternal age also has a decisive influence... ...  more of Tiemann-Boege Hunts Down Early Gene Material Mutations (Titel)

Tiemann-Boege: Winner of the OOE Forscherinnen Award 2015, Category “Gesundheit”

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[2015/05/20] Top recognition for the Johannes Kepler University (JKU) Linz at the 2015 Upper Austrian Research Awards for Women... ...  more of Tiemann-Boege: Winner of the OOE Forscherinnen Award 2015, Category “Gesundheit” (Titel)


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Membrane Transport, Peter Pohl

The research foci include transport of water, protons, other small molecules, and proteins through biological membranes. We thus exploit (i) primary cells or tissues, (ii) cultured cells that are genetically modified to overexpress the protein of interest or (iii) planar lipid bilayers or lipid vesicles both reconstituted with the purified membrane transporter. Purification occurs by overexpressing E.coli cells or yeast cells using affinity and size-exclusion chromatography.
      Our work aims to unravel molecular transport mechanisms. We specifically want to understand (i) the major determinants of single file water transport in potassium channels, aquaporins, artificial nanopores, (ii) the principles of water flux through secondary active transporters, (iii) the structural-functional relationship which allows the protein translocase to facilitate the passage of large molecules while maintaining the membrane barrier for small molecules, (iv) the mechanisms of lateral proton migration between two membrane proteins.

The proteins under investigation are genetically or chemically modified to test the functional importance of certain residues and to introduce fluorescent labels. We monitor protein function by implementing a wide variety of methods including: recordings of current through single channels and through channel ensembles, streaming and boundary potential measurements, particle electrophoresis, stopped- flow spectroscopy, fluorescence correlation spectroscopy, fluorimetry, confocal fluorescence microscopy, and scanning electrochemical microscopy.