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Biophysics Researchers: Constant Calcium Uptake Causes Cancer in Cells

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[2017/Nov/28] Dr. Irene Frischauf and Dr. Rainer Schindl made the cover of Science Signaling with their publication: Calcium regulates many vitally important functions in the body - if the calcium balance is defective, diseases like cancer develop... ...  more of Biophysics Researchers: Constant Calcium Uptake Causes Cancer in Cells (Titel)

Noteworthy Publication in Angewandte Chemie

[2016/Nov/06] Publication in Angewandte Chemie: "Detailed Evidence for an Unparalleled Interaction Mode between Calmodulin and Orai Proteins" ...  more of Noteworthy Publication in Angewandte Chemie (Titel)

Victoria Lunz - Genuinely Passionate about Research

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[February 2017] Photo credited to OÖ Nachrichten ...  more of Victoria Lunz - Genuinely Passionate about Research (Titel)

Tuning membrane protein mobility by confinement into nanodomains

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[2016/11/16] In cooperation with Prof. Peter Pohl (Institute of Biophysics, JKU), lead CBL researchers DI Andreas Karner and Dr. Johannes Preiner have developed a platform to study membrane proteins. ...  more of Tuning membrane protein mobility by confinement into nanodomains (Titel)


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Membrane Transport, Peter Pohl

The research foci include transport of water, protons, other small molecules, and proteins through biological membranes. We thus exploit (i) primary cells or tissues, (ii) cultured cells that are genetically modified to overexpress the protein of interest or (iii) planar lipid bilayers or lipid vesicles both reconstituted with the purified membrane transporter. Purification occurs by overexpressing E.coli cells or yeast cells using affinity and size-exclusion chromatography.
      Our work aims to unravel molecular transport mechanisms. We specifically want to understand (i) the major determinants of single file water transport in potassium channels, aquaporins, artificial nanopores, (ii) the principles of water flux through secondary active transporters, (iii) the structural-functional relationship which allows the protein translocase to facilitate the passage of large molecules while maintaining the membrane barrier for small molecules, (iv) the mechanisms of lateral proton migration between two membrane proteins.

The proteins under investigation are genetically or chemically modified to test the functional importance of certain residues and to introduce fluorescent labels. We monitor protein function by implementing a wide variety of methods including: recordings of current through single channels and through channel ensembles, streaming and boundary potential measurements, particle electrophoresis, stopped- flow spectroscopy, fluorescence correlation spectroscopy, fluorimetry, confocal fluorescence microscopy, and scanning electrochemical microscopy.