Lise Meitner Fellowship M1097
Project start: January 2010
We are using a technology called bead-emulsion amplification (BEA) to study mutations and recombination hotspots. With this technology we can analyze in parallel millions of single molecule PCR reactions. Single molecules are amplified on microscopic beads in isolation by compartments formed by an emulsion.
Product length, dye choice, and detection chemistry in the bead-emulsion amplification of millions of single DNA molecules in parallel
Tiemann-Boege I, Curtis C, Shinde DN, Goodman D, Tavaré S, Arnheim N.
Analytical Chemistry 81(14): 5770-6. Published: 2009.
Massively Parallel Haplotyping on Microscopic Beads for the High-Throughput Phase Analysis of Single Molecules.
Boulanger J, Muresan L, Tiemann-Boege I.
PLoS One, 7(4): e36064. Published: 2012.