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DK Nanocell

Logo DK Nanocell

We are taking part in the interdisciplinary graduate college called "Nanocell", with the project "Modelling of conformational transitions of fluorescent labelled proteins". ...  more of DK Nanocell (Titel)

Location Physics-Building

Location Physics-Building

Maps and directions to JKU. ...  more of Location Physics-Building (Titel)

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2017 - Julia Neuwirth

Two-Photon Excitation STED Microscopy

Research stay at the Bewersdorf Laboratory, and Cell Biology Department, Yale, USA
May 2017 – Oct 2017

Julia Neuwirth
Contact: vorname.nachname1(/\t)

I am currently studying Technical Physics as a master student at the institute of Applied Physics of the JKU under the direction of Prof. Thomas Klar. The topic of my thesis is "Improved STED-microscopy and -lithography using adaptive optics". Part of the research work of my thesis I conducted at the Department of Cell Biology in the Bewersdorf Laboratory at Yale university. There, they have expertise with respect to a novel microscopy technique called "Two-Photon Excitation STED Microscopy".


Abb.1: STED 

Resolution in microscopy, long considered limited to 1/3 of the wavelength, can be overcome by switching off the fluorescence in the outer rim of the excitation beam quickly enough. One possibility to inhibit such a spontaneous emission is to use STimulated Emission to Deplete (STED) the excited state.