Zur JKU Startseite
We investigate of axonal plasticity and the role of nanoscale interactions in highly synchronized neuronal behavior focusing on the axon initial segment (AIS), a critical site for action potential generation and neuronal excitability. Our previous work has contributed to an emerging field in axon biology, namely the role of the AIS as a homeostatic scaling domain that has a direct impact on neuronal excitability in the context of network changes in vivo. By utilizing advanced immunofluorescence microscopy, we examine rodent and human brain slices to explore the nanoscale organization of the AIS, its inner component and its direct interaction partners such as glial cells and axo-axonic synapses. A key focus of our work is the implementation of state-of-the-art imaging methods, including expansion microscopy, stimulated emission depletion (STED) microscopy, and other super-resolution techniques to dissect the molecular architecture of the AIS and its role in neuronal network dynamics.
Current Research Areas
Electrical Coupling at the AIS: We investigate the presence of connexin clusters along the AIS and their dynamic adaptation in different network states. Our findings suggest a link between these molecular assemblies and intra-axonal calcium stores known as cisternal organelles, providing new insights into how these structures might contribute to high-frequency neuronal synchronization and epileptogenesis.
Super-Resolution Imaging of Axonal Microdomains: Our group specializes in nanoscale imaging approaches, including STED microscopy and expansion microscopy, to resolve structural details of neuronal nanodomains. We explore how these microstructures interact with key molecular components both within the axon as well as in the direct vicinity to regulate neuronal function.
Human Brain Tissue Studies: By directly examining human brain slices, we aim to uncover conserved mechanisms of AIS plasticity and compare them to findings from our work in rodent models. Understanding these processes in the human brain is crucial for bridging the gap between basic neuroscience and clinical applications.
