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Cytometry

The Core Facility Cytometry offers a wide range of highly specialized services in the field of cellular analysis and sorting for biomedical research. Equipped with state-of-the-art flow cytometry and cell sorting instruments, it assists researchers in the examination and characterization of cell populations, immunophenotyping, cell cycle analysis, and cell function studies. In addition to training on equipment and various software, the Core Facility Cytometry also provides consultations in sample preparation, project planning, as well as analysis and interpretation of data.

Devices

Cell Analyzer

Our Core Facility is equipped with three state-of-the-art flow cytometers, each featuring 3 lasers (405, 488, 640 excitation wavelengths), up to 13 fluorescence channels, and with a spectral cytometer offering up to 38 fluorescence channels.

Spectral detection provides advantages such as simultaneous use of dyes like BV421, Super Bright 436, and eFluor® 450, APC and Alexa Fluor® 647, optimized detection of weak fluorescence markers through autofluorescence correction, and the combination of two side scatters (488/405 SSC).

After successful completion of the required training and approval by the Core Facility staff, these instruments are also available for independent use by researchers.

  • 3 lasers (405nm, 488nm, 638nm)
  • 13 fluorescence detection channels
    • 405nm Laser:
      450/45 BP, 525/40 BP, 610/20 BP, 660/10 BP, 780/60 BP
    • 488nm Laser:
      525/40 BP, 585/42 BP, 610/20 BP, 690/50 BP, 780/60 BP
    • 638nm Laser:
      660/10 BP, 712/25 BP, 780/60 BP
  • Single measurement in 5ml (12x75mm) or 1.5ml tubes
  • High-throughput measurements using plate loader (96-well)

CytoFLEX V5-R3-B5 Beckman Coulter, opens a file in a new window

  • 3 Laser (405nm, 488nm, 638nm)
  • 13 13 Fluorescence detection channels
    • 405nm Laser:
      450/45 BP, 525/40 BP, 610/20 BP, 660/10 BP, 780/60 BP
    • 488nm Laser:
      525/40 BP, 585/42 BP, 610/20 BP, 690/50 BP, 780/60 BP
    • 638nm Laser:
      660/10 BP, 712/25 BP, 780/60 BP
  • 32 Tube Standard Carousel

DxFLEX V5-R3-B5 Beckman Coulter, opens a file in a new window

  • 3 Laser (405nm, 488nm, 642nm)
  • 11 Fluorescence detection channels
    • 405nm Laser:
      Blue-V (450/45 nm), Green-V (525/30 nm), Yellow-V (583/26 nm), Red-V (695/50 nm), NIR-V (785/70 nm)
    • 488nm Laser:
      Green-B (525/30 nm), Yellow-B (583/26 nm), Red-B (695/50 nm), NIR-B (785/70 nm)
    • 642nm Laser:
      Red-R (661/14 nm), NIR-R (785/70 nm)
  • Single measurement in microcentrifuge tubes
  • High-throughput measurements using plate loader (96-well)

Guava®easyCyte™12HT Cytek, opens a file in a new window

  • 3 Laser (405nm, 488nm, 640nm)
  • 38 Fluorescence detection channels, 2 SSC, 1 FSC
  • Measurement of the complete emission spectrum from 420nm to 829nm.
    • 405nm Laser:
      428/15 BP, 443/15 BP, 458/15 BP, 473/15 BP, 508/20 BP, 525/17 BP, 542/17 BP, 581/19 BP, 598/20 BP, 615/20 BP, 664/27 BP, 692/28 BP, 720/29 BP, 750/30 BP, 780/30 BP, 812/34
    • 488nm Laser:
      508/20 BP, 525/17 BP, 542/17 BP, 581/19 BP,
      598/20 BP, 615/20 BP, 661/17 BP, 679/18 BP,
      697/19 BP, 717/20 BP, 738/21 BP, 760/23 BP,
      783/23 BP, 812/34 BP
    • 640nm Laser:
      661/17 BP, 679/18 BP, 697/19 BP, 717/20 BP,
      738/21 BP, 760/23 BP, 783/23 BP, 812/34 BP
  • Single measurement in 5ml (12x75mm) tubes

Cytek™ Northern Lights V16-B14-R8, opens a file

Cell Sorter

1. BioRad S3e Benchtop Sorter

The BioRad S3e Benchtop Sorter, equipped with a few fluorescence detectors and user-friendly technology, simplifies cell sorting. It allows simultaneous sorting of up to two subpopulations and depositing single cells in various formats.

2. BD FACSAria™Fusion High-End Sorter

The BD FACS Aria Fusion, featuring five lasers (355nm, 405nm, 488nm, 561nm, 640nm) and 18 fluorescence channels, enables complex high-speed cell sorting, high-purity sorting, deposition in various formats, and simultaneous sorting of up to four subpopulations.

Operation of the cell sorters is primarily conducted by Core Facility personnel. However, particularly regarding the use of the S3e Benchtop Sorter, there is an opportunity for the Core Facility to grant independent usage authorization to individual researchers with sufficient experience and completion of the required training.

  • 3 Lasers (405nm, 488nm, 561nm)
  • 4 Fluorescence detection channels
  • FL1 447/60 BP, FL2 525/30 BP, FL3 586/25 BP, FL4 600 LP)
  • 2-way sorting
  • Nozzle Size: 100µm
  • 5ml (12x75mm) sample tubes
  • Collection of sorted cells in 5ml tubes, multi-well strips, and microscope slides

S3e Cell Sorter Bio Rad, opens a file in a new window

  • 5 Lasers (355nm, 405nm, 488nm, 561nm, 640nm)
  • 18  Fluorescence detection channels
    • 355nm Laser (3 Detectors):
      A: 740/35 BP, 690 LP, B: 450/50 BP, 410 LP, C: 379/28 BP
    • 405nm Laser (6 Detectors):
      A: 780/60 BP, 750 LP, B: 710/50 BP, 690 LP, C: 660/20 BP, 630 LP,
      D: 610/20 BP, 595 LP, E: 525/50 BP, 505 LP F: 450/50 BP
    • 488nm Laser (2 Detectors):
      A: 695/40 BP, 655 LP, B: 530/30 BP, 502 LP
    • 561nm Laser (4 Detectors):
      A: 780/60 BP, 735 LP, B: 670/14 BP, 630 LP, C: 610/20 BP, 600 LP, D: 582/15 BP
    • 640nm Laser (3 Detectors):
      A: 780/60 BP, 755 LP, B: 730/45 BP, 690 LP, C: 670/30 BP
  • 4-way sorting
  • Nozzle Size: 70µm, 85µm, 100µm, 130µm
  • Collection of sorted cells in 5ml tubes, multi-well plates, and microscope slides

BD FACSAria™ Fusion 2-3-6-4-3_ACDU, opens a file in a new window

Services

  • Flow cytometric analyses
    • up to 13 fluorescence channels
  • Spectral cytometric analyses
    • up to 38 fluorescence channels
  • Sorting of heterogeneous cell populations
    • under temperature-controlled conditions
  • High-throughput analyses
    • in a 96-well format
  • Consultation on project planning
    • instrument setup, multi-color panel design, controls, etc.
  • Consultation on analysis and interpretation of data
[Translate to Englisch:] Edeltrau Raml bei der Arbeit

Contact

Name

Position

 Phone E-mail Address
Dr.in Anna-Maria Lipp Head of Core Facility

0664 9601459

 anna-maria.lipp@jku.at
Edeltraud Raml Biomedical Analyser 0732 2468 3091 edeltraud.raml@jku.at

Gallery of analysis photos

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[Translate to Englisch:] Analysenfoto
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Johannes Kepler University Linz

Altenberger Straße 69

4040 Linz, Austria

Telephone +43 732 2468 0
Fax +43 732 2468 4929
Contact