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Imaging

The Core Facility Imaging provides a broad instrumental base for acquiring and analyzing microscopic data in 2D, 3D, and 4D. Both fluorescent and (non-fluorescent) histochemically stained and unstained samples can be recorded, ranging from macroscopic to molecular resolution. A well-coordinated range of instruments, including fluorescence microscopes, confocal microscopes, STED, and electron microscopy provides necessary instrumentation. The Core Facility Imaging not only offers training on the devices but also consultations from sample preparation to data analysis.

 

Devices

Equipment:

  • Thermionic electron source
  • Constant-power C-TWIN objective lens
  • Ceta family of 16-Mpixel CMOS Cameras
  • Next-generation Panther STEM detector
  • Velox analysis software
  • Automatic cryo box
  • TEM tomography data acquisition software
  • Tomography holder
  • Inspect3D Software
  • Velox Software
  • TIA Software
  • Maps 3 for S/TEM with correlative workflow
  • Amira Software

Room: Huemerstraße B020

Talos STEM, opens a file in a new window

Equipment:

  • Motorized reflected light filter wheel
  • Motorized universal condensor
  • Filter: DAPI, FITC, CY3, CY5, CY7, CFP, YFP
  • LED-Light source
  • Halogen Lamp
  • UplanFLN 4x/0.13 Phl /- FN 25.5
  • UplanFLN 10x/0.30 Ph1 /- FN 26.5
  • LUCPlanFLN 20x/0.45 Ph1 /0-2 FN 22
  • UplanSApo 20x/0.85 Oil /- FN 26.5
  • LUCPlanFLN 40x/0.60 Ph2 /0-2 FN22
  • UplanSApo 100x/1.40 Oil /0.17 FN 26.5
  • cellSens Software

Room: Blutzentrale OG3/W/06

Olympus IX73, opens a file in a new window

Equipment:

  • Laser modules LM URGB
  • Colibri 5 RGB-UV LED light source
  • Filter: 90 HE LED
  • EC Plan-Neofluar 2.5x/0085
  • P Apo 10x/0.45
  • P Apo 20x/0.8
  • Plan Apochromat 63x/1.4 Oil DIC
  • LD LCI P Apo 40x/1.2 ImmKorrDIC
  • Axiocam 305 mono R2
  • Scanner GaAsP-PMT f/LSM 900
  • LSM 900 Airyscan 2 Detector 63x
  • Zeiss Zen Software Package
  • Zen Module Sample Finder
  • Zen Module Airyscan

Room: Huemerstr. B020

LSM900, opens a file

Equipment:

  • Ex.Laser: 405, 485, 561, 640
  • STED Laser: 775
  • 3 ADP Detectors
  • 1 Matrix detector
  • Easy 3D STED unit
  • Adaptive optics
  • UPLX APO 60X 0, NA 1.42 Oil
  • UPL X APO 40x 0
  • UPLX APO 20x
  • UPLFLN 10x2
  • IX3-SSU stage
  • Z-drift comp. Unit
  • DIC contrast imaging comp.
  • Lightbox Software

Room: LAB 305

STED Facility Line, opens a file
 

Equipment:

  • BioTek BioSpa Live Cell Analysis System
  • AutoScratch Wound Making
  • CO2 and O2 gas controller
  • Peltier Cooling Module
  • Dispenser/Injector Module
  • 1.25x Objective top
  • 5x Objective Inverse
  • 10 x Objective Inverse
  • 20x Objective inverse
  • Filter cubes DAPI, GFP, Texas Red, CY5
  • Gen5 Software

Room: LAB 308

Cytation7 , opens a file

Equipment:

  • 96 well plate insert
  • HCPL Fluotar 10x /0.3
  • HC PL APO 20x/0.75 imm corr CS2
  • HC PL APO 63x/1.30 Glyc corr CS2
  • HC PL APO 40x/1.25 Glyc corr CS2
  • Laser 405 nm
  • Leica White Laser (485nm - 685nm)
  • Filtercubes: 405, GFP
  • LAS X Software
  • 3x Power HyD detectors

Room: LAB 315


Stellaris, opens a file

 

Equipment: 

  • Holder frame KM Multiwell plates
  • CLICK-IN P "35" Petri dishes
  • CLICK-IN P all Chambered systems
  • HC PL FLUOTAR 10x/0.32 PH1
  • HC PL FL L 20x/0.40 CORR PH1 1
  • HC PL FLUOTAR 40x/0.80 PH2 1
  • HC PL FLUOTAR 63x/1.10 IMM CORR PH3
  • HC PL APO 40x/1.10 W motCORR
  • LED8 LED Light Source
  • Filter cube DFT51010
  • Filter cube CYR71010
  • LAS X Software

Room: LAB 315

Leica Thunder System , opens a file

Equipment:

  • Upright fluorescence microscope
  • Light source Colibri 7 Typ R[G/Y]CBV-UV
  • Filter 90 HE LED
  • Plan Apochromat 10x/0.45 Ph1
  • P Apo 20x/0.8 PH2
  • Plan-Apo 40x/1.3 Oil DIC (UV)VIS-IR
  • Plan-Apochromat 63x/1.4 Oil DIC
  • W N-ACHROMAT 20x/0.5
  • W PLAN-Apochromat 40x/1.0 DIC VIS-IR
  • Camera Axiocam 705 mono
  • Camera Axiocam 208 color
  • ZEN Software

Room: LAB 306

Zeiss Axio Imager, opens a file

Services

  • Fluorescence microscopy
  • Transmission electron microscopy (TEM)
  • Confocal laser scanning microscopy (LSM)
  • Stimulated Emission Depletion (STED) microscopy
  • Phase-contrast microscopy and DIC
  • High-throughput imaging and live-cell analysis
  • Automated image analysis
  • 3D imaging
  • Software-assisted image processing and analysis
  • Project-specific script/software development
[Translate to Englisch:] Dr. rar. net. Arndt Rohwedder bei der Arbeit

Contact

Name

Position

Phone

E-mail Address
Dr. rer. nat. Arndt Rohwedder Head of Core Facility 0732 2468 3096
0664 602468417		 arndt.rohwedder@jku.at
Dr.in Sabine Knipp University Assistant 0664 602468415 sabine.knipp@jku.at
Mag.a Ancuela Andosch Biomedical Analyst   ancuela.andosch@jku.at
Logo Softwarefirma Huygens
Leica Stellaris
Mikroimage , opens a file
[Translate to Englisch:] Testbild
LSM900
Microimage , opens a file
[Translate to Englisch:] Microimage
STED
Microimage , opens a file
[Translate to Englisch:] Microimage STED
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Johannes Kepler University Linz

Altenberger Straße 69

4040 Linz, Austria

Telephone +43 732 2468 0
Fax +43 732 2468 4929
Contact