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Next-Generation Sequencing (NGS) & Molecular Biology

The Core Facility for Next-Generation Sequencing (NGS) and Molecular Biology offers a broad spectrum of technologies in the field of genetic analysis. The advanced sequencing platforms enable genome and transcriptome sequencing at DNA and RNA levels, as well as the sequencing of short reads and ultra-long reads. In addition to training on the devices, the Core Facility for NGS and Molecular Biology also provides guidance in sample preparation, data analysis, and interpretation of results.



The NextSeq 2000 Sequencer by Illumina generates up to 360 Gb per run, with an error rate of < 0.34%. It supports read lengths of up to 600 bases. Our NextSeq 2000 is equipped with an integrated DRAGEN module, allowing for fast and precise secondary analyses, including variant calling.

NextSEQ2000, opens a file in a new window

The MinION Mk1C by Oxford Nanopore Technologies generates up to 48 Gb per Flow Cell, with an error rate of < 0.5%. The unique Nanopore Technology enables the sequencing of ultra-long reads, exceeding 4 Mb. Additionally, selective sequencing can be activated upon request, allowing molecules containing predefined sequences to be promptly discarded or selectively sequenced.

MinION Mk1C, opens an external URL in a new window

The quality control of DNA or RNA samples is conducted using automated electrophoresis on our 2100 Bioanalyzer from Agilent Technologies.

The nCounter Sprint Profiler by NanoString Technologies enables rapid RNA sequencing in under 24 hours for the entire workflow. Up to 800 transcripts can be selectively quantified in this process. Additionally, custom panels of up to 55 targets can be included upon request.

Using our RT-PCR systems from Bio-Rad Laboratories, we specifically quantify DNA or RNA in qPCR experiments. Both 94-well and 384-well systems are supported in this process.


  • Genome- and transcriptome-based sequencings
  • Short-read sequencing
  • Ultra-long-read sequencing
  • Single Cell Analyses
  • Quality control of DNA/RNA samples
  • Quantification of DNA/RNA
[Translate to Englisch:] Molekularbiologe bei der Arbeit





E-mail Address

Dr. Johannes Pröll

Head of Core Facility

0732 2468 3090


David Demmel, MSc Biologit 0732 2468 3092 david.demmel@jku.at